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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Cell-free fat extract promotes tissue regeneration in a tissue expansion model

Fig. 5

a, b Western blotting analyses of the vascular endothelial cell growth factor receptor (VEGFR), epidermal growth factor receptor (EGFR), VEGF, EGF, collagen type 1 (COL-1), collagen type 3 (COL-3), and basic fibroblast growth factor (bFGF) in expanded tissue. a Representative western blot results from three independent experiments are shown. b Cell-free fat extract (CEFFE) treatment significantly increases the expression of VEGFR, EGFR, COL-1, and COL-3. The expression of VEGF, EGF, and bFGF is decreased by CEFFE in the expanded tissue. Density values are expressed in arbitrary units. c, d Western blotting analysis of COL-1; COL-3; matrix metalloproteinase (MMP)-1, − 3, and – 9; and tissue inhibitor of metalloproteinases 1 (TIMP-1) in the expanded tissue. c Representative results from three independent experiments are shown. β-actin was used as the loading control. d Density values are expressed in arbitrary units relative to β-actin. Data are expressed as means ± SD. *p < 0.05

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