Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: AICAR and nicotinamide treatment synergistically augment the proliferation and attenuate senescence-associated changes in mesenchymal stromal cells

Fig. 5

Effects of AICAR, NAM, and AICAR+NAM on the intracellular signaling pathways of AMPK, mTORC1, and autophagy. a MSCs at passage 5 were treated with AICAR, NAM, and AICAR+NAM for further five passages. Immunofluorescence staining of the study groups at P10 using anti-phospho-AMPK (AMPK-α1-pThr183 and AMPK-α2-pThr172) antibody, anti-phospho-p70 S6 Kinase (Thr389) antibody, and anti-LC3B antibody. The nuclei were visualized using DAPI (scale bar = 100 μm). b Representative data of phospho-AMPK, Phospho-p70 S6 kinase (Thr389), and LC3B immunofluorescence, calculated as the number of positive cells per total number of cells. Each bar indicates mean ± SD (*p < .05; **p < .01; ***p < .001; ****p < .0001). Abbreviations: AICAR, 5-aminoimidazole-4-carboxamide ribonucleotide; NAM, nicotinamide

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512