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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration

Fig. 7

Effect of PEMF-induced conditioned medium (CM) on inflamed chondrocytes. Inflammation was induced in chondrocytes with 5 ng/ml IL-1β for 24 h. CCM or PCM was administered to chondrocytes 24 h post inflammation induction. Real-time PCR analysis of cartilaginous and inflammatory markers and NOS activity were performed at 24 h (plain bars) and 48 h (hatched bars) post-supplementation with CM and normalized to GAPDH, presented as a fold-change relative to the level in non-treated (day 0) chondrocytes. Data shown represent means ± SD, n = 6 from 2 independent experiments. * denotes significant differences compared to the non-inflamed controls (no IL-1β treatment); # denotes significant differences compared to the inflammation controls (IL-1β alone treatment) and @ denotes significant differences compared to respective CCM. Col 2 = type II collagen; COX-2 = cycloxigenase-2; IL-1β = interleukin-1β; IL-6 = interleukin-6; MMP-13 = metalloproteinase 13; NOS = nitric oxide synthase

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