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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Multipotency of mouse trophoblast stem cells

Fig. 4

Differentiation of CD117+ TSCs into alveolar epithelial cells of lung in vitro. a Phase-contrast images showing morphologic change of cells before (Undiff, top row, left) and after differentiation (Diff, top row, right) in epithelial cell differentiation medium on air-liquid interface for 14 days. These differentiated cells were immunostained for AQP5 (red, middle row, left), SPC (red, middle row, right), and ZO-1 (red, bottom row, left). b Bar graph showing the quantitation of qRT-PCR for AQP5 and SPC in fold change comparing differentiated (Diff, black bars) to undifferentiated cells (Undiff, open bar). *P < 0.05 compared to their undifferentiated cells, n = 5 for each group

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