Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility

Fig. 1

Schematic representation of the study design. From all the animals of the previous study, one donor (black), four recipients of MSC-naïve (all mismatched, dark gray), and six recipients of MSC-primed (three halfmatched, black; three mismatched, dark gray) were selected to assess humoral response against allogeneic mesenchymal stem cells (MSCs) based on their equine leukocyte antigen (ELA) haplotypes. Peripheral blood lymphocytes (PBLs), unstimulated MSCs (MSC-naïve), and MSCs pre-stimulated with tumor necrosis factor alpha and interferon gamma (MSC-primed) of the same ELA haplotype than the donor were used as target cells. Sera collected from the selected recipients at different time-points (T0, pre-administration of corresponding MSCs; T1, 1 week after first MSC administration; T2, 3 weeks after first MSC administration—just before the second MSC administration; T3, 1 week after second MSC administration; T4, 90 days after second MSC administration) were tested neat, 1:2 and 1:16 diluted against all the three types of target cells using two-stage microcytotoxicity assays

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512