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Table 1 Summary and description of the variables of the study

From: Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility

DonorRecipients
ELA haplotypesTarget cellsGroupELA haplotypesTime-points of sera harvestingSera dilution
ZAR07/ZAR08PBLs1 (control)
MSC-naïve (unstimulated)
MSC-primed (5 ng/ml TNFα and 5 ng/ml IFNγ for 12 h)
Naïve-mismatched:
• Injected with MSC-naïve
• ELA-mismatched with donor (0/2 haplotypes shared)
N = 4
ZAR01/ZAR21
ZAR01/ZAR02
ZAR01/ZAR02
ZAR01/ZAR06
T0 = pre-administration of MSCs
T1 = 1 week after 1st MSC administration
T2 = 3 weeks after 1st MSC administration (just before 2nd administration)
T3 = 1 week after 2nd MSC administration
T4 = 90 days after 2nd MSC administration
Neat (no dilution)
Dilution 1:2
Dilution 1:16
Primed-halfmatched:
• Injected with MSC-primed
• ELA-halfmatched with donor (1/2 haplotypes shared)
N = 3
ZAR07a/ZAR09
ZAR07/ZAR10
ZAR07/ZAR14
Primed-mismatched:
• Injected with MSC-primed
• ELA-mismatched with donor (0/2 haplotypes shared)
N = 3
ZAR03/ZAR12
ZAR03/ZAR16
ZAR09/ZAR13
  1. ELA haplotypes of the donor selected and type of target cells; groups of recipients according to MSCs received and their haplotypes (when possible, sharing of one haplotype among recipients was used as criteria to select more homogeneous groups: naïve-mismatched recipients all shared the haplotype ZAR01; primed-halfmatched recipients shared the haplotype ZAR07 among them and with the donor - one of the recipients presented the variation ZAR07a; two primed-mismatched recipients shared the haplotype ZAR03 and the other one did not share any haplotype); time-points at which sera was collected from recipients and sera dilutions assessed. MSCs mesenchymal stem cells, ELA equine leukocyte antigen, TNFα tumor necrosis factor alpha, IFNɣ interferon gamma, T time, PBL peripheral blood lymphocyte. 1PBLs were obtained from a different horse but with the same ELA haplotypes than the donor selected