Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility

Table 2 Primers used for amplification of horse intra-MHC microsatellites

	Locus		Dye	Primer sequence	Allele range (bp)	Reference
MHC Class I	UMNJH-38	F′	FAM	TGTGTGTGCACCTGTCCTTT	156–165	Sadeghi et al., 2018 [18]
	UMNJH-38	R′		GATGGGAGGGAATGAGGAAT	156–165	Sadeghi et al., 2018 [18]
	COR110	F′		TTTGGTCTTTGCAGGTATGG	194–221	Tseng et al., 2010 [19]
	COR110	R′	VIC	TCTCCCTTCCTCTTTGTTCC	194–221	Tseng et al., 2010 [19]
MHC Class III	ABGe 9019	F′	FAM	CTGAGAGAGACAGCATTTGTGG	297–320	Sadeghi et al., 2018 [18]
	ABGe 9019	R′		GAAAGGTGTCTCCATTCTTGCT	297–320	Sadeghi et al., 2018 [18]
	UNMe65	F′	AT550 (NED)	TCGCAAAACCCACAGACTAC	247–269	Sadeghi et al., 2018 [18]
	UNMe65	R′		TTCTCCTTTCCTTCCACTCC	247–269	Sadeghi et al., 2018 [18]
MHC Class II	ABGe 9030	F′	AT565 (PET)	CCAGCAGACCTGCAAGAGTA	205–221	Sadeghi et al., 2018 [18]
	ABGe 9030	R′		AGCATGAGAGCCATGAAGGT	205–221	Sadeghi et al., 2018 [18]
	EQMHC1	F′	AT532 (VIC)	ATGCATACCGGGAAAGACAG	180–196	Sadeghi et al., 2018 [18]
	EQMHC1	R′		AGAGACTTCAGTCTCTGTGGTG	180–196	Sadeghi et al., 2018 [18]
	COR112	F′		TTACCTGGTTATTGGTTATTTGG	236–268	Tseng et al., 2010 [19]
	COR112	R′	NED	TCACCCACTAAATCTCAAATCC	236–268	Tseng et al., 2010 [19]
	COR113	F′		TGTTTAGAACTCGCCAGGAG	260–276	Tseng et al., 2010 [19]
	COR113	R′	FAM	TCATCAGTTCCTTGCCTAGC	260–276	Tseng et al., 2010 [19]
	UM011	F′		TGAAAGTAGAAAGGGATGTGG	165–180	Tseng et al., 2010 [19]
	UM011	R′	FAM	TCTCAGAGCAGAAGTCCCTG	165–180	Tseng et al., 2010 [19]
	COR114	F′		TCAAAATCCACACTCCCTTC	234–255	Tseng et al., 2010 [19]
	COR114	R’	PET	TCCATAAAGAGTGGGACACTG	234–255	Tseng et al., 2010 [19]

Primers (F′, forward; R′, reverse), dye, sequence, allele range (base pair) and reference

ISSN: 1757-6512