Fig. 7
Evaluation of MN preservation and glial reactivity in the lumbar spinal cord after combined BMC injection. a, b, c. Representative photographs of lumbar spinal cord sections showing L4 MNs of a WT, b BMC-treated SOD1G93A mice, and c SHAM SOD1G93A mice. d Quantification of MN survival after injection of BMCs in spinal cord and hindlimb muscles. Dotted line indicates the mean number of MNs in wild type mice. e, f, h, i Illustrative micrographs showing immunoreactivity against Iba-1 (green, microglia) or GFAP (red, astrocytes) at 16 weeks of age in BMCs-treated SOD1G93A mice and SHAM SOD1G93A mice. Quantification of g microglia and j astrocytes after injection of BMCs (n = 6) or vehicle (n = 4) in SOD1G93A mice. Error bars indicate SEM