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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice

Fig. 4

IGF-II overexpression affected telomerase activity, telomere length, and cell cycle regulator expression during PSC differentiation. a–c The telomerase activity (a) at differentiation days 2, 10, and 24 and telomere length at differentiation day 24. (b, c) was detected in cardiomyocytes derived from various clones. Data in a and c are presented as the fold change relative to the untransfected ESC line D3 at day 0. *p < 0.05 vs. all other groups. d Cell derivatives from various clones were collected at differentiation day 24. Fetal, neonatal, juvenile, and adult hearts were enzymatically digested, followed by cardiomyocyte purification. 1 × 105 cardiomyocytes originated from different sources were analyzed by Western blot to detect protein expression of cell cycle regulators, proliferating cell nuclear antigen and cyclin D2. e Quantification of d. The expression level of each band was normalized to that of β-actin. Data are presented as a logarithmic scale of the fold change relative to the expression in the pME 18s D3 group. ∆p < 0.05 vs. pME 18s PSCs or ESCs, but p > 0.05 vs. juvenile cardiomyocytes

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