Fig. 3

Osteogenic activity of MSC treated with CM. a ALP activity in MSC treated or not (−) for 48 h with CM from MΦ_GM and MΦ_M activated (CM_GM+ and CM_M+, respectively) or not (CM_GM− and CM_M− , respectively) with LPS and further incubated in osteogenic medium (OM) for 14 days. b. Alizarin red staining and quantification in MSC treated or not with CM from macrophages and further incubated in OM for 14 days (upper panel) or 21 days (lower panel). Data in b are relative to those measured in untreated MSC incubated in growth medium (GrM), which were given an arbitrary value of 100. *p < 0.05 between the indicated conditions