Fig. 4
Expression of bone-related genes in MSC treated with CM. aRUNX2, COL1A1, and ALPL mRNA levels in MSC treated or not (−) for 48 h with CM from MΦGM and MΦM activated (CMGM+ and CMM+, respectively) or not (CMGM− and CMM−, respectively) with LPS. Data are relative to those measured in untreated MSC, which were given an arbitrary value of 1. bRUNX2, COL1A1, and ALPL mRNA levels in MSC treated or not (−) for 48 h with CMGM+ or CMM+ and further incubated in osteogenic medium (dark gray) for the indicated time points. Data are relative to those measured in untreated MSC incubated in growth medium (light gray) for 3 days, which were given an arbitrary value of 1. *p < 0.05 between the indicated conditions