Fig. 7

Morphology, adhesion, viability, and migration of MSC treated with TNF-α or IL-10. a Optical microscope images showing the morphology of MSC treated or not (−) for 48 h with the indicated doses of TNF-α or IL-10, after crystal violet staining. b Ratio of cell length to width as a measure of cell elongation. c Number of MSC treated or not with TNF-α or IL-10 and incubated in growth medium (GrM) for the indicated time points. d Viability of MSC treated or not with TNF-α or IL-10 and further incubated in GrM for the indicated time points. The data are relative to those measured in each group on day 1, which were given an arbitrary value of 100. e Migration capacity of MSC seeded inside the area confined by squared silicone barriers, treated or not with TNF-α or IL-10 and allowed to migrate for 5 days. Optical microscope images of cells stained with crystal violet. *p < 0.05 between the indicated conditions