Fig. 7

The osteogenic differentiation of hMSCs in which miR-765 was knocked down and at the same time as a BMP/Smad signaling inhibitor was added to disrupt Smad1/5/9 phosphorylation. Alizarin Red S staining was employed as a means of assessing matrix mineralization. Scale bar, 50 μm. a By adding a BMP/Smad signaling inhibitor, the osteogenic differentiation of hMSCs in which miR-765 was also knocked down was associated with decreased matrix mineralization relative to cells in which miR-765 was knocked down but no inhibitor was added. b Differences in mineralization staining intensity were significant between shRNA+inhibitor and shRNA groups. Data are expressed as means ± SD (X ± SD, n = 3). **P < 0.01 vs. shRNA+inhibitor, respectively