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Fig. 9 | Stem Cell Research & Therapy

Fig. 9

From: miR-765 inhibits the osteogenic differentiation of human bone marrow mesenchymal stem cells by targeting BMP6 via regulating the BMP6/Smad1/5/9 signaling pathway

Fig. 9

miR-765 suppresses BMP6/Smad1/5/9 signaling to inhibit osteogenesis. Western blotting was used to measure p-Smad1/5/9 levels, with β-actin as a loading control. a miR-765 overexpression was associated with reduced Smad1/5/9 phosphorylation upon osteogenic differentiation. b miR-765 knockdown significantly enhanced p-Smad1/5/9 levels. c By adding a BMP/Smad signaling inhibitor to reduced p-Smad1/5/9 levels. BMP6/Smad1/5/9 was shown to be downstream of miR-765, with miR-765 controlling the activation of this pathway. Data are expressed as means ± SD (X ± SD, n = 3). **P < 0.01 vs. negative controls or shRNA+inhibitor, respectively. Note: NC: negative control; OE: miR-765 overexpression; sh: shRNA; sh+inh: sh+inhibitor

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Stem Cell Research & Therapy

ISSN: 1757-6512