Fig. 3

MiR-144 targets and negatively regulates CCNE1 and CCNE2. a, b Prediction of upstream targeted miRNAs of CCNE1 and CCNE2. c Comparison between upstream miRNAs of CCNE1 and CCNE2 and differentially expressed miRNAs predicted from GSE102286. d Expression of miR-144 in GSE102286. e The expression of miR-144 in NSCLC tissues and adjacent normal tissues tested by RT-qPCR. *p < 0.05 vs. the adjacent normal tissues. f The expression of miR-144 in NSCLC cell lines identified by RT-qPCR. *p < 0.05 vs. the HBE cell line. g The binding sites of miR-144 on CCNE1 and CCNE2 predicted by the bioinformatics websites (http://www.microrna.org/microrna/home.do). h The binding of miR-144 to CCNE1 and CCNE2 confirmed by dual luciferase reporter assay. *p < 0.05 vs. A549 cells transfected with NC. i The expression of CCNE1 and CCNE2 assessed by RT-qPCR. j The expression of CCNE1 and CCNE2 detected by western blot analysis. *p < 0.05 vs. A549 cells transfected with NC mimic. ^#p < 0.05 vs. A549 cells transfected with NC inhibitor. Comparisons between two groups were analyzed by unpaired t test. Data among multiple groups were analyzed by one-way ANOVA, followed by a Tukey post hoc test. The experiment was repeated in triplicate