Fig. 4

Overexpression of miR-144 attenuates NSCLC cell proliferation and colony formation and reduces the number of S phase-arrested cells. a Cell proliferation detected by EdU assay (× 200). b Number of colony formation assessed by colony formation assay. c Cell cycle progression measured by flow cytometry. d The expression of proliferation-related factors determined by western blot analysis. *p < 0.05 vs. A549 cells transfected with NC mimic. ^#p < 0.05 vs. A549 cells transfected with NC inhibitor. The above measurement data were expressed as mean ± standard deviation. Comparisons between two groups were analyzed by unpaired t test. The experiment was repeated in triplicate