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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Targeted silencing of miRNA-132-3p expression rescues disuse osteopenia by promoting mesenchymal stem cell osteogenic differentiation and osteogenesis in mice

Fig. 1

Osteogenic differentiation of BMSCs induced by an osteogenic medium. a Gene expression of osteogenic differentiation markers Runx2, Osx, and Alp was detected by qRT-PCR after osteogenic induction. b ALP protein activity was detected after osteogenic induction. c Protein expression of RUNX2 and OSX was detected by Western blot analysis and quantified using ImageJ software. d The calcific nodules of extracellular matrix were detected by Alizarin Red staining after 21 days of osteogenic induction. e Expression level of miRNA-132-3p was detected as BMSCs differentiated along the osteogenic lineage in vitro. Values are shown as mean ± SD, n = 3 in each group. *P < 0.05, **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512