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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Targeted silencing of miRNA-132-3p expression rescues disuse osteopenia by promoting mesenchymal stem cell osteogenic differentiation and osteogenesis in mice

Fig. 2

Function of miRNA-132-3p on osteogenic differentiation of BMSCs. BMSCs were transfected with the mimic (mimic-132), inhibitor (antimir-132), or their negative control (miR-NC) and were then induced to osteogenic lineage. a The expression of miRNA-132-3p after 4 days of osteogenic induction. b Gene expression of Runx2, Osx, and Alp after 4 days of osteogenic induction (compared to miR-NC). c The protein activity of ALP after 4 days of osteogenic induction. d Protein expression of RUNX2 and OSX after 4 days of osteogenic induction. e The calcific nodules of the extracellular matrix after 21 days of osteogenic induction. Values are shown as mean ± SD, n = 3. *P < 0.05, **P < 0.01. NS, no significant

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