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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Targeted silencing of miRNA-132-3p expression rescues disuse osteopenia by promoting mesenchymal stem cell osteogenic differentiation and osteogenesis in mice

Fig. 4

Downregulation of miRNA-132-3p partly attenuates the effects of mechanical unloading on BMSC osteogenic differentiation in vitro. BMSCs were transfected with the inhibitor of miRNA-132-3p for 6 h and were then exposed to gravitational unloading for 48 h. After the unloading exposure, BMSCs were cultured with osteogenic medium for 4 days. a miRNA-132-3p expression in BMSCs was analyzed after its antimiR was transfected. b Gene expression of Runx2, Osx, and Alp. c The protein activity of ALP was analyzed. d Protein expressions of RUNX2 and OSX were analyzed. Values are shown as mean ± SD, n = 3. *P < 0.05, **P < 0.01. NS, no significant

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Stem Cell Research & Therapy

ISSN: 1757-6512