Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Role and therapeutic effects of skeletal muscle-derived non-myogenic cells in a rat myocardial infarction model

Fig. 4

Therapeutic potential of NMC or myoblast cell-sheets. a Cell sheets consisting of myoblasts or NMCs were transplanted by left thoracotomy into nude rats subjected to left coronary artery ligation 2 weeks before transplantation. Sham rats served as controls. a LVEF, LVESD, and LVEDD were serially assessed by transthoracic echocardiography. Myocardial fibrosis (b), capillary density (d), and ratio of ILB-4/αSMA double-positive vessel in ILB-4 cells (e) in the peri-infarct area 4 weeks after cell-sheet transplantation were assessed by Masson’s trichrome staining and immunohistochemistry, respectively (scale bars: b 1000 μm, d 50 μm, e 20 μm). c Expression of fibrosis-related gene was assessed by real-time PCR. f Expression of human-specific gene in host myocardium was assessed by real-time PCR. LVEF, left-ventricular ejection fraction; LVESD, left-ventricular end-systolic dimension; LVEDV, left-ventricular end-diastolic dimension; ILB-4, isolectin-B4; αSMA, α-smooth muscle actin. *p < 0.05, **p < 0.01

Back to article page