Fig. 3

The effect of LV-FGF-2⁺-hGMSC-CM on the expression of angiogenesis-related factors in HUVECs. HUVECs were pretreated with hGMSC-CM, LV-vector⁺-hGMSC-CM, or LV-FGF-2⁺-hGMSC-CM for 3 days (negative control group was cultured by ECM) and then re-cultured by ECM for 7 to 10 days. The mRNA and protein expressions of PLGF, SCF, and VEGFR2 were determined by qRT-PCR (a–c) and western blot (d–i). The VEGFR2 protein expression was also detected by immunofluorescence staining (VEGFR2, red fluorescent signals; DAPI, blue signals; × 400 magnification) (j, k). ns = no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001