Fig. 3
From: Standardized GMP-compliant scalable production of human pancreas organoids
Comparison of RSPO1 CM and RSPO1 molecule cultures. a Representative bright-field images of hPOs in culture with RSPO1 CM and R (RSPO1 recombinant molecule). Scale bar, 500 μm. b, c Mean (±SEM) diameter and area at day 7 in each culture condition (≥ 100 organoids measured) Two-way analysis of variance (ANOVAs) followed by Newman-Keuls post hoc tests for multiple comparisons was used. d, e Mean (±SEM) glucose consumption and lactate production of hPOs in culture with CM and R at different time points (n = 3). Two-way analysis of variance (ANOVAs) followed by Newman-Keuls post hoc tests for multiple comparisons was used. f Gene expression analysis of pancreatic markers. Mean expression values (±SEM) were normalized to those of hPOs in CM (n = 3) and Student’s t tests was used