Fig. 4
From: Standardized GMP-compliant scalable production of human pancreas organoids
Characterization of hPOs after cryopreservation. a Representative bright-field images of fresh hPOs (fresh) and hPOs after thawing (thawed). Scale bar, 500 μm. b, c Mean (±SEM) diameter and area of fresh and thawed hPOs at day 7 (≥ 100 organoids measured). Two-way analysis of variance (ANOVAs) followed by Newman-Keuls post hoc tests for multiple comparisons was used. d, e Mean (±SEM) glucose consumption and lactate production of fresh and thawed hPOs at study time points (n = 3). Two-way analysis of variance (ANOVAs) followed by Newman-Keuls post hoc tests for multiple comparisons was used. f Mean (±SEM) expression of pancreatic markers (n = 3) and Student’s t tests was used