Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

Fig. 1

Generation of NANOG tdTomato knock-in reporter positive piPS cells. A Overview of the strategy for constructing NANOG tdTomato knock-in positive cells. The target sequences of NANOG on Chr1 and Chr5 are identical. B Analysis of NANOG tdTomato knock-in positive colonies. (a) Before colonies were picked. (b) After colonies were picked and regrown. Scale bar 50 μm. C FACS analysis of NANOG tdTomato knock-in positive cells. (a) FACS tdTomato positive cells. (b) Fluorescence micrograph of cells sorted by FACS. Scale bar 200 μm. D Sanger sequencing results for the target locus and insert junctions for chromosomes 1 and 5. E Immunocytological analysis of NANOG tdTomato knock-in positive cells. NANOG tdTomato knock-in positive cells were positive for FLAG and NANOG protein; WT PC-iPS cells were negative for FLAG and heterogeneous for NANOG. Scale bar 25 μm

Back to article page