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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

Fig. 3

Effects of base medium components on NANOG regulation. A Experimental design for testing the effects of medium components, cytokines, and inhibitors on NANOG tdTomato regulation. B Light and fluorescence microscopy images showing expression of tdTomato NANOG in cells grown in LCDMV from which individual medium components have been removed. (a) When DIM was removed from the media, light microscopy showed that the cells differentiated to a flatted state, cells in all other media were dome-shaped; (b) fluorescence microscopy revealed that tdTomato NANOG was expressed in all media. Scale bar 200 μm. C Flow cytometric analysis of tdTomato cells grown in LCDMV from which individual medium components have been removed. D RT-PCR analysis of NANOG mRNA expression in tdTomato cells grown in LCDMV from which individual medium components have been removed. ****< 0.0001; ***< 0.001; **< 0.01, *< 0.5; ns, not significant

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Stem Cell Research & Therapy

ISSN: 1757-6512