Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

Fig. 4

Activation of NANOG expression by various cytokines. A Images obtained using optical and fluorescence microscopy showing NANOG expression in NANOG tdTomato knock-in positive cells grown in the presence of different cytokines. Growth in T1 medium alone was used as a negative control. (a) Light microscopy showed that cells treated with bFGF had a flattened and differentiated morphology, cells treated wilt other cytokines were dome-shaped. Scale bar 200 μm; (b) examination by fluorescence microscopy showed that only cells exposed to Activin A expressed tdTomato. Scale bar 100 μm. B Flow cytometric analysis of NANOG tdTomato knock-in positive cells cultured in the presence of different cytokines. C RT-PCR analysis of RNA harvested from NANOG tdTomato knock-in cells cultured in the presence of different cytokines. ****< 0.0001; ***< 0.001; **< 0.01, *< 0.5; ns, not significant

Back to article page