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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

Fig. 6

Rescue of NANOG expression after SB431542 treatment. A Optical and fluorescence microscopy showing NANOG expression in NANOG tdTomato knock-in positive cells grown in T1 alone, T1 + Activin A, and T1 + SB4316542. (a) Light microscopy showed that all cells were dome-shaped; (b) examination by fluorescence microscopy showed that cells treated with Activin A expressed more fluorescence than the controls, while those treated with SB431542 expressed no fluorescence. Scale bar 100 μm. B Immunocytological analysis of NANOG tdTomato knock-in positive cells grown in T1 + Activin A and T1 + SB4316542. FLAG and NANOG proteins were expressed in the Activin A-treated cells, while they were not detectable in T1 + SB4316542. Scale bars 20 μm. C Rescue of NANOG tdTomato expression after SB431542 treatment. (a) Light microscopy showed that NANOG tdTomato-positive PC-iPS cells cultured in T1 or T1 + Activin A were dome-shaped. (b) NANOG tdTomato expression was rescued only in cells cultured in T1 + Activin A. Scale bar 200 μm. D Immunocytological analysis shows that NANOG expression was seen only in cells cultured in T1 + Activin A after SB4316542 treatment. Scale bar 20 μm

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