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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: A cytokine screen using CRISPR-Cas9 knock-in reporter pig iPS cells reveals that Activin A regulates NANOG

Fig. 7

RNA-Seq analysis of cells treated with Activin A and SB431542. a Volcano plot showing expression values for genes in cells treated with Activin A versus SB431542. Genes represented by gray dots did not meet the criteria for differential expression. (log2[fold-change ≥1]; adjusted p value ≤ 0.05). (n = 3). b Heat map of the clustering analysis for differentially expressed genes in cells treated with Activin A versus SB431542. Adjst p<0.05 (n = 3). c Heat map showing expression of Activin/SMAD target genes. Adjst p<0.05 (n = 3). d Expression [log2(FPKM+1)] of Activin A/SMAD pathway target genes. e RT-PCR analysis of SMAD2/3 target gene expression. f Western blot analysis of selected proteins from cells grown with Activin A and SB431542. GAPDH was used as a loading control. g Model of the mechanism for Activin A regulation of NANOG. ****< 0.0001; ***< 0.001; **< 0.01, *< 0.5; ns, not significant

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