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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

Fig. 2

Induction of iPSC to retinal progenitors and their characterization. a TC-1133 hiPSC colony. b hiPSC expressing more than 95% OCT4. c Formation of embryoid bodies from iPSC and their induction to retinal progenitors. d–g Immunofluorescence images representing the protein expression at day 20 before selection. d Neuroectodermal markers PAX6, Nestin, SOX1, and SOX2. e Retinal commitment markers RX, LHX2, CRX, and OTX2. f Neural markers FOXG1 and GFAPΔ. g Expression of OCT4 and Ki67. h Gene quantification using real-time PCR of selected markers in day 20 retinal progenitors and day 25 RPE progenitors and photoreceptor progenitors after rosette selection represented as fold change with respect to the undifferentiated iPSC. Images and graphs are representative of a minimum of three independent experiments. Scale bars represent 100 μm

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