Fig. 2

Cell function. a Population doubling time (PDT). b Colony-forming Assay. c, d Proliferation assessed with BrdU staining and Images merged, BrdU, and DAPI. e Cell cycle distribution. f Reactive oxygen species (ROS) expression. g, h Apoptosis assessed with TUNEL staining of the sample and the positive control (DNAse I). Statistical significance is indicated with asterisks: * p value ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001. No asterisks represent p > 0.05. N = 3 for each group (=cells from 3 different patients per group); triplicates per biological sample. Graph: Mean with SEM