Fig. 8

The effects of RvE1 on odonto/osteogenic differentiation in LPS-stimulated hDPSCs. Staining and quantitative detection of ALP activity in hDPSCs under incubation of RvE1 with or without 1 μg/mL LPS treated for 7 and 14 days (A). Alizarin red staining of the hDPSCs after culture in RvE1 with or without 1 μg/mL LPS treated for 14 and 21 days (B). Relative gene expression levels of DMP1, DSPP, and BSP of hDPSCs after culture in RvE1 with or without 1 μg/mL LPS treated for 7, 14, and 21 days (C). Control, PBS with osteogenic-induced medium group; LPS, 1 μg/mL LPS with the osteogenic-induced medium group; RvE1, 100 nM RvE1 with the osteogenic-induced medium group; LPS + RvE1, 1 μg/mL LPS and 100 nM RvE1 with the osteogenic-induced medium group. *P < 0.05, **P < 0.01, ***P < 0.001. The results are mean ± standard deviation of triplicate measurements from three independent experiments