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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Adult tissue-resident stem cells—fact or fiction?

Fig. 1

Stem cells in adult pancreas. a Stem cell enrichment from adult pancreas by centrifuging at higher speed. Adult mouse pancreas was subjected to enzymatic digestion for preparing a single cell suspension. b Upon centrifugation at 250g, large numbers of cells of different sizes with abundant cytoplasm and pale stained nuclei were observed in the cell smear after H&E staining. c On further centrifuging the supernatant at 1000g, putative, spherical stem cells with high nucleo-cytoplasmic ratio and darkly stained nuclei are clearly visualized. These stem cells can be further characterized by flow cytometry, immuno-localization and RT-PCR as published earlier [9]. b and c have been reproduced from our original article [9] with permission from Springer Nature

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