Fig. 1
From: MSC-NTF (NurOwn®) exosomes: a novel therapeutic modality in the mouse LPS-induced ARDS model
Analysis of MSC and MSC-NTF cells and their derived EVs. a FACS analysis of the MSC markers CD73, CD90, and CD105 on MSC and MSC-NTF cells. b Nanoparticle tracking analysis of naïve Exo MSC and Exo MSC-NTF. Exo MSC had a median size of 146 nm, and Exo MSC-NTF had a median size of 114 nm. c Transmission electron microscopy image of Exo MSC and Exo MSC-NTF. Scale bar represents 100 nm. d MACSPlex exosome kit FACS analysis of exosome expression of tetraspanins (CD9, CD63, CD81), MSC (CD44, CD29), and isotype controls (REA Ctrl, mIgG1 Ctrl) in Exo MSC and Exo MSC-NTF. e, f Immunomodulatory activity of the sEVs as determined by inhibition of IFNγ and TNFα secretion by activated PBMCs. Cell culture supernatant ELISA was performed following incubation with EVs from four independent donors relative to untreated activated PBMCs. Mean ± SEM, *p < 0.05 paired t test