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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: NAMPT/SIRT2-mediated inhibition of the p53-p21 signaling pathway is indispensable for maintenance and hematopoietic differentiation of human iPS cells

Fig. 2

Effects of NAMPT or SIRT2 knockdown on the proliferation and survival of human iPS cells. a mRNA expressions of NAMPT (left) and SIRT2 (right) in iPS cells transduced with shNAMPT (left) or shSIRT2 (right) LV were evaluated. iPS cells transduced with shCtrl LV was used as control. Fold changes relative to control are shown (***p < 0.001, ****p < 0.0001). b Cell growth of iPS cells transduced with shNAMPT (left) or shSIRT2 (right) lentivirus (LV) were quantified using trypan blue dead cell exclusion. iPS cells transduced with shCtrl LV was used as control. Fold changes relative to control are shown (***p < 0.001, ****p < 0.0001). c Apoptosis was analyzed in shNAMPT (left) or shSIRT2 (right) LV-transduced iPS cells using Annexin V assay. iPS cells transduced with shCtrl LV were used as control. Diagrams show the percentage of Annexin V-positive apoptotic cells (**p < 0.01, ***p < 0.001, ****p < 0.0001). d Cell cycle was analyzed in shNAMPT (left) or shSIRT2 (right) LV-transduced iPS cells using BrdU assay. iPS cells transduced with shCtrl LV was used as control. Diagrams show the percentage of cells in S phase (**p < 0.01)

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Stem Cell Research & Therapy

ISSN: 1757-6512