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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: NAMPT/SIRT2-mediated inhibition of the p53-p21 signaling pathway is indispensable for maintenance and hematopoietic differentiation of human iPS cells

Fig. 6

Knockdown of p53 attenuates the growth inhibition in FK866- or AC93253-treated iPSCs. a mRNA expression of p53 in iPS cells transduced with shp53 LV was evaluated. iPS cells transduced with shCtrl LV was used as control. Fold change relative to control is shown (****p < 0.0001). b Western blot analysis of p53 protein expression in shp53 LV-transduced iPS cells. iPS cells transduced with shCtrl LV was used as control. GAPDH was used as loading control. Representative WB images are depicted. c, d Growth of iPS cells transduced with control shRNA or p53-specific shRNA and subsequently treated with DMSO, FK866 (1 nM or 2 nM), or AC93253 (50 nM or 100 nM) was evaluated over time with the IncuCyte S3 Live-Cell Analysis System

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