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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Generation of dyskeratosis congenita-like hematopoietic stem cells through the stable inhibition of DKC1

Fig. 1

Molecular implications associated with the inhibition of DKC1 in human hematopoietic stem and progenitor cells. Cord blood CD34+ cells were transduced with specific anti shRNA-LVs and maintained in liquid cultures for 5–8 days (see details in the “Materials and methods” section). a Decreased expression of DKC1 gene after CD34+ cell transduction with specific shRNA-LVs (7 independent experiments were conducted; n = 7). DKC1 expression levels in cells transduced with DKC1 shRNA LVs represent relative values of those obtained in cells transduced with the scrambled shRNA LV. Raw data are shown in Suppl. Table 2. b Decreased expression of TERC after CD34+ cell transduction with specific shRNA-LVs (n = 7). Raw data are shown in Suppl. Table 2. c Representative analysis of a telomeric repeat amplification protocol (TRAP). Internal control is marked by the black arrow, negative control was performed with buffer (NC) and non-transduced cells were used as control. d Analysis of the telomerase activity after transduction with shRNA LVs (n = 3). Data are expressed as mean ± SEM. Asterisks indicate significant differences determined by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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