Fig. 3

Analysis of the in vitro growth properties and in vivo repopulating ability of DKC1-interfered CD34⁺ cells. a Analysis of the cell expansion analyzed 2 weeks after ex vivo incubation of transduced cells in liquid culture (n = 5). b Relative cell expansion in comparison with cells transduced with the scrambled shRNA-LV (n = 6). c Analysis of the clonogenic potential of CD34⁺ cells transduced with DKC1-shRNA LVs and scrambled shRNA-LVs (n = 8). d Analysis of the repopulation potential of CD34⁺ cells transduced with scrambled shRNA-LV (orange dots) or DKC1-shRNA LVs (blue dots). The proportion of human CD45⁺ cells in the BM of recipient mice was analyzed at 1–3 months post-transplantation (mpt). Data are expressed as mean ± SEM. Asterisks indicate significant differences determined by Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)