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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Combined macromolecule biomaterials together with fluid shear stress promote the osteogenic differentiation capacity of equine adipose-derived mesenchymal stem cells

Fig. 4

Evaluation of the osteogenic markers expression. MSCs were cultivated in osteogenic differentiation (OD) medium in the presence of MC, B30, and B30Str biomaterials under static and mechanical FSS culture condition (N = 8 per experimental group). Cells were osteogenic induced without adding biomaterials served as control (NC). Cells grown in parallel in basal medium (BM) were used as non-induced control. The levels of Runx2 and alkaline phosphatase expression at day 14 and day 21 were quantified using RT-qPCR. a Relative Runx2 expression at day 14 under static culture condition. b Relative Runx2 expression at day 21 under static culture condition. c Relative ALP expression at day 14 under static culture condition. d Relative ALP expression at day 21 under static culture condition. e Relative Runx2 expression at day 14 under FSS condition. f Relative Runx2 expression at day 21 under FSS culture condition. g Relative ALP expression at day 14 under FSS culture condition. h Relative ALP expression at day 21 under FSS culture condition. The expression was normalized to non-induced cells in BM using 2−ΔΔCt method [34]. GAPDH was used as an endogenous reference. The data presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001

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