Fig. 4

miR-199a-3p promoted the LPS-induced inhibition of PC12 cell differentiation by regulating downstream factors of NGF/TrkA. a, b Representative western blot images and quantitative analysis of NF-H, β-tubulin-III, and Neu-N gene expressions in LPS- and NGF-stimulated PC12 cells pre-transfected with different doses of miR-199a-3p mimics. Data are represented as average value of 3 independent experiments. c, d Representative western blot images and quantitative analysis of western blots of p-Akt and p-Erk expression (n = 3). Data are represented as mean ± SD. e, f Representative immunofluorescence images and quantitative analysis of cell neurite growth (red arrow) and neuronal marker expression (NF-H, β-tubulin-III, and Neu-N) in LPS- and NGF-stimulated PC12 cells pretreated with different doses of miR-199a-3p mimics (n = 3). Data are represented as mean ± SD. Scale bar = 50 μm. *p < 0.05; **p < 0.01, LPS lipopolysaccharide, NGF neuronal growth factor