Fig. 5

miR-145-5p promoted LPS-induced inhibition of PC12 cell differentiation by regulating downstream factors of NGF/TrkA. a, b Representative images of western blots and quantitative analysis of NF-H, β-tubulin-III, and Neu-N gene expressions in LPS- and NGF-stimulated PC12 cells pre-transfected with different doses of miR-145a-5p mimics. Data are represented as average value of 3 independent experiments. c, d Representative western blot images and quantitative analysis of p-Akt (I) and p-Erk (J) expression in LPS- and NGF-stimulated PC12 cells pre-transfected with different doses of miR-145-5p mimics (n = 3). Data are represented as mean ± SD. e, f Representative immunofluorescence images and quantitative analysis of cell neurite growth (red arrow) and neuronal marker expression (NF-H, β-tubulin-III, and Neu-N) in LPS- and NGF-stimulated PC12 cells pretreated with different doses of miR-145a-5p mimics (n = 3). Data are represented as mean ± SD. Scale bar = 50 μm. *p < 0.05; **p < 0.01. LPS lipopolysaccharide, NGF neuronal growth factor