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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Umbilical mesenchymal stem cell-derived exosomes facilitate spinal cord functional recovery through the miR-199a-3p/145-5p-mediated NGF/TrkA signaling pathway in rats

Fig. 6

miR-199a-3p and miR-145-5p targeted the Cblb and Cbl genes, respectively. Predicted binding sites of miR-199a-3p (a) and miR-145-5p (b) on Cblb and Cbl mRNAs. c–e Cblb and Cbl gene expressions in PC12 cells transfected with different doses of miR-199a-3p/145-5p mimics and inhibitors assayed by western blot and QRT-PCR (n = 3). Data are represented as beanplot exhibiting values from 3 independent experiments. Luciferase reporter assay of the first predicted binding site (f) and the second predicted site (g) of miR-199a-3p in HEK293 cells. Data are represented as mean ± SD. h Luciferase reporter assay of the predicted binding site of miR-145-5p in HEK293 cells (n = 3). Data are represented as mean ± SD. *p < 0.05; **p < 0.01; #p > 0.05. WT wild type, MT mutant, QRT-PCR quantitative real-time polymerase chain reaction

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