Fig. 8

Function of exosomal miR-199a-3p/145-5p in vivo in SCI rats. a Relative miR-199a-3p/145-5p expression levels in SCI and SCI of MSC-Exo- and Exo-K-treated rats at 3 DPI (n = 3). Data are represented as mean ± SD. b Representative images and quantitative analysis of TrkA in SCI, SCI treated with Exo, and SCI treated with Exo-K rats at 3 DPI (n = 3). Data are represented as mean ± SD. c, d Representative images and quantitative analysis of TUNEL staining in sham-operated rats, SCI rats, SCI rats treated with Exo, and SCI rats treated with Exo-K 3 DPI (n = 3). Scale bar = 500 μm, scale bar = 100 μm. Data are represented as mean ± SD. e BBB scores of the four groups of rats (sham, SCI, SCI + Exo, and SCI + Exo-K) on the 1st, 3rd, 5th, 7th, 14th, 21st, 28th, and 35th DPI (n = 10/group). Data are represented as mean ± SD. f, g Quantitative analysis and representative images of the lesion size (HE staining) in the four groups of rats at 35 DPI (n = 10). Data are represented as mean ± SD. Scale bar = 1000 μm. h Representative MRI images of the lesion area (red arrow) of rats at 35 DPI. *p < 0.05; #p > 0.05. SCI, spinal cord injury; DPI, day post injury; MSC-Exo, MSC-derived exosomes; Exo-K, MSC-Exo with miRNA inhibition; BBB, Basso, Beattie and Bresnahan; HE, hematoxylin-eosin