Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: MicroRNA-205 mediates endothelial progenitor functions in distraction osteogenesis by targeting the transcription regulator NOTCH2

Fig. 3

MiR-205 suppresses the expression of NOTCH2 and controls angiogenesis. a Sequence alignment of mature miR-205 and the canine NOTCH2 3′UTR. b PCR analysis of NOTCH2 mRNA expression levels during the DO process. c NOTCH2 expression in ECFCs and serum samples from the peripheral blood. d, e RNA and protein levels of miR-205, NOTCH2, and angiogenic cytokines were detected by PCR (d) and Western blotting (e). f VEGF secretion from transfected ECFCs was measured via ELISA. g Dual-luciferase reporter assays were used to measure relative luciferase activity for wild-type (WT) or mutant (MUT) NOTCH2 reported following miR-205 co-transfection. Data are means ± S.D (N.S, no significance; *P < 0.05, **P < 0.01 and ***P < 0.001; n = 3 independent experiments)

Back to article page