Fig. 4

NOTCH2 plays a vital role in ECFC angiogenesis. PCR (a) and WB (b) analysis of the RNA and protein levels of NOTCH2, VEGF, and bFGF in ECFCs after NC and NOTCH2 inhibitor transfection. c ELISA analysis of VEGF secretion from ECFCs after transfection. Tube formation (d, e), Transwell (f, g), wound healing (h, i), and proliferation (j) assays revealed that inhibiting NOTCH2 impairs angiogenesis. k, l CAM angiogenesis assays revealed that when injected with ECFCs in which NOTCH2 was inhibiting, new chick embryo vessel density decreased. Data are means ± S.D. (N.S, no significance; ^#P > 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001; n = 3 independent experiments)