Fig. 5

MiR-205 regulates ECFC function via inhibiting NOTCH2. PCR (a) and WB (b) analyses of the RNA and protein levels of NOTCH2, VEGF, and bFGF in ECFCs after transfection with dual-NC, NOTCH2i-NC + miR-205i, or both inhibitors. c VEGF secretion from ECFCs after transfection was measured via ELISA. The results of proliferation (d), tube formation (e, f), Transwell (g, h), and wound healing (i, j) assays revealed that miR-205 was able to reverse the angiogenic activity that was impaired by NOTCH2 inhibitor. k, l Representative photographs of capillaries in CAM samples. Data are means ± S.D. (N.S, no significance; ^#P > 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001; n = 3 independent experiments)