Fig. 6

Inhibiting miR-205 accelerates osteogenesis and remodeling during DO. A Gross observational result. B Histological analyses revealed that inhibiting miR-205 was able to accelerate new bone formation. C CBCT results from control, negative control, and miR-205 inhibitor groups following 0, 2, 3, and 4 weeks of consolidation. Few calluses were observed in the distraction gap at the end of the distraction phase (DO14) in these groups. The amount of mineralized callus increased over time, with more callus being evident in the miR-205 inhibited group. The distraction gap was fully bridged at the end of the consolidation phase (bar = 10 mm). D Quantitative analyses of CBCT results revealed that the Hounsfield unit (HU) values were significantly increased during DO and were higher in the miR-205 inhibition group relative to the other groups