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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: RETRACTED ARTICLE: Effects of miR-672 on the angiogenesis of adipose-derived mesenchymal stem cells during bone regeneration

Fig. 1

miR-672 regulates ADSC angiogenesis. a qPCR analysis of intracellular VEGF and miR-672 levels in ADSCs treated with or without BMP2 at different time points. b Western blotting analysis of intracellular VEGF levels in ADSCs treated with BMP2 at different time points. c qPCR analyses of angiogenic factors related genes VEGF, SDF-1, ANGPT, and FGF in ADSCs transduced with Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC for 0 days, 3 days, and 7 days. d Blood vessel formation analysis of ADSCs transduced with Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC. e Western blotting analysis of angiogenic factors related genes VEGF, SDF-1, FGF, and ANGPT in ADSCs transduced with Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC for 0 days, 3 days, and 7 days. f Nb Junctions, Nb nodes, Nb meshes, and Tot. length were all analyzed about the blood vessel formation. Scale bar 200 μm for d. All data represent averages from three independent experiments. Statistical significances were determined by unpaired Student’s t test using GraphPad Prism Version 8. *P < 0.05, **P < 0.01, ***P < 0.001

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