Fig. 2

TIMP2 is negatively regulated by miR-672. a Western blotting analysis showed the expression of TIMP2 following the transduction of Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC. b qPCR showed the mRNA expression levels of TIMP2 following the transduction of Lenti-miR-672, Lenti-as-miR-672, and Lenti-miR-NC. c Schematic diagram showed the wild type and mutant binding sites of miR-672 located in the TIMP2 3′-UTR. d The dual luciferase reporter assay showed the relative luciferase expression levels. The firefly luciferase activity data was normalized to the Renilla luciferase activity. All data represent averages from three independent experiments. Statistical significances were determined by unpaired Student’s t test using GraphPad Prism Version 8. **P < 0.01