Fig. 3

TIMP2 knockdown promotes the effects of the miR-672. a qPCR analyses of the expression of the angiogenic factor-related genes VEGF, SDF-1, ANGPT, and FGF in ADSCs transduced with Lenti-miR-NC or Lenti-as-miR-672, with or without TIMP2 knockdown. b Western blotting analyses of VEGF, SDF-1, ANGPT, and FGF protein in ADSCs transduced with Lenti-miR-NC or Lenti-as-miR-672, with or without TIMP2 knockdown. c Quantitative analysis of western blot relative greyscale normalized to β-actin. d Blood vessel formation analysis of ADSCs transduced with Lenti-miR-NC or Lenti-as-miR-672, with or without TIMP2 knockdown. e–h Nb Junctions, Nb nodes, Nb meshes, and Tot. length were all analyzed about the blood vessel formation. Scale bar 200 μm for d. All data represent averages from three independent experiments. Statistical significances were determined by unpaired Student’s t test using GraphPad Prism Version 8. **P < 0.01