Fig. 1

CD9 is enriched in AML-LSC population. a The Venn diagram compares the number of specifically expressed and shared differentially expressed genes among the three AML LSCs sequencing chips. Fifty-five common differentially expressed genes were analyzed in the MRD microarray by the Venn diagram. b The expression levels of 8 genes related to membrane proteins. c Flow cytometry was used to detect the expression of CD9 in the AML cell lines THP-1 and HL-60. d Gating strategy for comparing the expression of CD9 on AML blasts (CD3⁻CD19⁻CD45^lowSSC^low) and LSC-enriched CD34⁺CD38⁻ populations (CD3⁻CD19⁻CD45^lowSSC^lowCD34⁺CD38⁻). e The expression of CD9 on AML blasts and LSC-enriched CD34⁺CD38⁻ populations from the bone marrow of patients with AML (n = 5). f CD9 expression in the normal bone marrow